![]() ![]() Also if you are not comfortable ordering online, we are always happy to take your order by phone. MAGIC TILT Trailer Bunks and Bunk Hardware TRAILER PARTS SUPERSTORE® sells MAGIC TILT factory replacement boat trailer bunks, bunk brackets and bunk mounting hardware. There are no dumb questions – don’t hesitate to call us. If you do not see a part listed that you need, please contact or call us and we will help you 813.630.5695. We stock: Accessories, Axles, Bearings, Boat Guides, Brakes, Bunks/Brackets/Carpets, Cross Members, tongue Material, Swings, Fasteners, Fenders, Hubs, Lighting, Rollers, Springs, Spring Mounts, Tires and Spare Mounts, Jacks and Couplers, Winch Posts, and Winches.īe sure to also read our helpful articles in our News & Blog section such as How to Measure Different Types of Trailer Springs, Types of Axles, and Trailering with BrakesĪt Boat Trailer Parts Place – Tampa Florida, we also welcome calls for any questions you may have to ensure that you get the correct part for your trailers. GALVANIZED TRAILERS MAGIC TILT BOAT TRAILERS Magic Tilt has been building quality boat trailers since 1953. If you have difficulty accessing features or functions on this website, email us at and we will work with you to provide the information you seek and/or call our customer service line at 72. If you don’t see what you need, please call our parts department at (813) 630-5695. Magic Tilt has taken steps and is devoting resources to promote website accessibility. 7GA Galvanized Cross members Heavy-duty hot-dipped galvanized drop center cross member with 7 gauge nominal wall thickness. We do however sell thousands of other parts. We sell new and pre-owned Boat Trailers, Cargo Trailers, Flatbed Trailers. This is a listing of our most popular parts. Tropic Trailers is a dealership with locations in Fort Myers and Marianna. Welcome – We are the #1 source for all your trailer parts needs. Check out our Captain’s Club Rewards Program for additional discounts and savings on your next order at Wholesale Marine.Boat Trailer Parts Place – Tampa Florida. ![]() Contact us Monday through Friday 9:00 AM to 6:00 PM at 87. We offer same-day flat rate shipping and our prices can’t be beat. We can answer any questions and help place your order for the perfect boat trailer guide. LESLIE'S TRAILER PARTS AND ACCESSORIES Complete line of frailer Parts and. Our experienced customer service representatives share your love of boating and are ready to help. HELP WANTED Foremen for Merry - Co - Rounds, Ferris Wheels, Tilt-a-Whirls. Have questions about which trailer guide is right for your boat, give us a call. Want to add a little extra protection to your current guide set up? Hyperlite offers trailer guide pads that slip over your guide post and are available in many sizes and colors. Smiths Trailer guide-ons with LED can help make navigating the dark easy. Ever had to launch a boat at night? This can be a difficult task during the daylight hours, but launching at night adds an extra element of risk, using C.E. Whether you are doing it solo or with the help of a fellow passenger these guides will make your job a snap. Just like a trailer is essential to getting your boat to and from the water, many boaters would also consider boat trailer guides a key component to a successful loading or launching. Wholesale Marine Carries Boat Trailer Guides for Every Craft axles BOAT TRAILER all brands magic tilt and more BOAT TRAILER PARTS PLACE TAMPA FLORIDA1 SOURCE FOR ALL YOU TRAILER PARTS NEEDS. We offer all types of guides from post trailer guide ons, floating trailer guide-ons, bunk board trailer guide-ons, and trailer guide pads to offer the best solution for your needs. These guides are available for all boat styles and PWC’s. Check out C.E Smith or Sea Sense line of trailer guide-on in LED lighted on non-lighted versions. 52.50 Qty: Add to Cart MAGIC TILT 25-1/4' Double Eye Trailer Leaf Spring (3 leaves / 2,000 lb. capacity factory direct replacement leaf spring. Want to look like a professional at the boat ramp but also avoid damage during launch and loading. MAGIC TILT 20' Double Eye Trailer Leaf Spring (3 leaves / 1,500 lb.) PJ1350 Stock 5428181 MAGIC TILT 1,500 lb. From bunk style carpeted boat guides, guide protectors, trailer guide on light kits and brackets we have the products in stock to get the job done. No matter what your boat trailer guide needs, we have you covered. ![]() Wholesale Marine has the trailer guide and trailer padding systems in stock to ensure your launch and trailering efforts go smoothly. These guides are attached to your trailer and help guide your vessel onto the trailer to avoid wear and tear on your boat and trailers and avoid costly accidents. Boat trailer guides are a great way to protect your boat and your trailer from expensive repairs. ![]()
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![]() The same just can’t be said for alcoholics and drug addicts. If you force a person with Bipolar Disorder to take the right medications, they’re going to have a reaction to those meds whether they like it or not and (hopefully) get better. It’s the same with many other types of mental illnesses. If someone has cancer and doesn’t think they have cancer or refuses treatment, in theory you could hold them down or knock them out and force them to have the treatments, and the cancer would be cured (hopefully) no matter what that person thought. That’s so different from other types of illnesses, even other mental illnesses. If someone doesn’t want to stop, they’re not going to get sober no matter what you do, and it doesn’t really matter if they’re a true addict or not at that point. It could be a ton of other reasons, but the reasons don’t really matter. I’ve felt that before, especially in the early days of my disease. It could be because they can’t imagine how they could live without it. ![]() They may never have tried to stop because they saw no reason to, especially if they weren’t have severe consequences (severe enough, in their opinion, to make them want to stop).īut they might also be like me and still not want to stop, which means they’re alcoholics/addicts and either don’t know it because they’ve never tried to stop or just don’t care if they are and wouldn’t stop anyway. There are certainly people out there who use and drink a lot like I do but have the ability to stop or moderate on their own, and for whatever reason, they don’t exercise it.įor many people who use or drink heavily, they may just not want to stop. Does this addict want to stop getting high?.If you’re reading this, and you have a friend or family member who is an addict or an alcoholic who can’t stop, there are 2 important questions to ask: If the Addict/Alcoholic Doesn’t Want to Stop, You Have a Different Problem So that answers the question of why addicts can’t stop using drugs, but there’s an important assumption being made here: we’re assuming the addict/alcoholic wants to stop. It was like nothing I’ve ever experienced. I just couldn’t defeat the obsession to drink and use. I truly wished that I wasn’t doing those things. I wasn’t hurting my family and friends because I wanted to. But while it’s true my body is doing these things, for years I didn’t want to be doing it. After all, our hands are the ones lifting the bottles to our lips, putting the needles in our veins, putting the powders up our noses. I know that it looks like the addict/alcoholic is doing this on purpose. That’s why a true addict can’t stop using. I was using against my will, and it was horrific. I found myself buying coke despite the fact that I absolutely hated using it. I would find myself in liquor stores after I swore for the 100th time that I’d never drink again. I would find myself dialing the numbers of drug dealers after changing my number and getting a new phone. I watched myself do these horrible things and couldn’t make it stop. ![]() I’ve never felt more out of control than when I was using. It was disgusting, and I hated it, and I did it anyway. I’ve found myself walking into Staples to buy a can of air duster to suck down because I couldn’t get any alcohol or other drugs. It means I’ll use anything to get high because I cannot stop thinking about it. It means I cry while getting high because I don’t want to do it anymore, because I feel like a self-destructing robot with no free will. It means I walk out of rehabs wanting to stop, only to use that day. ![]() I use and drink despite the consequences. It means I use and drink far beyond the point where it feels good or is fun. ![]() That means I drink more than I intend to. When I am forced to stop (I go to jail or rehab or run out), I cannot get the idea out of my head no matter what I do. Once I start, that compulsion to keep going cannot be stopped. I have a compulsion to keep drinking and using once I put a substance in my body, and I have a compulsion to get more once I sober up. Just as a person with diabetes is physically different from me-my body makes the insulin I need, theirs doesn’t-my addict body is physically different from the average person’s: We call addiction a disease for a reason: drugs and alcohol do something to my body that they don’t do to other people. If they would, and they truly wanted to, I promise you that they could (some don’t want to stop, and that’s a different story). You’ll hear a lot of reasons why addicts can’t stop doing drugs and why alcoholics can’t stop drinking, but the main reason they can’t stop is because they literally cannot stop-their brains won’t allow it. ![]() ![]() ![]() The company is not limited to just a specific type of merchandise which makes it even better.įrom building materials to clothing to sporting goods, you can buy everything wholesale here. If you are looking for a place in Illinois for liquidation pallets of high quality, your search might just end here. A majority of the merchandise you find here has never been used and is in a brand new stage as they never made it to their designated outlets. They specialize in acquiring overstocks and excess merchandise from a wide range of industries. is one of the most prominent liquidators in Chicago. Outside of the quality of goods, for their fast shipping and responsive customer support, Shorewood deserves to be mentioned among the best liquidators in Illinois.Īddress: 21460 SW Frontage Rd, Shorewood,c 60404, United Statesīased in Illinois, American Liquidation Inc. They mostly sell through online auctions hosted on their website which any registered user can participate in. Shorewood Liquidators is a reselling company that works mostly on the movement of liquidated or returned merchandise pallets of various retailers from all over the country. The pallets are pre-packaged and nobody at the Rogue goes through them or alters what’s inside them.Īddress: 309 S Staley Rd Ste B, Champaign, IL 61822, United States You can order pallets online too as they have a delivery service operating across various states. Customers can always visit their warehouse to personally check on items they are looking for. Rogue always labels its products so you know what you are getting into. ![]() They are one of the best sources of liquidation pallets in Illinois. If you do not want to participate in auctions, you can order a full truckload or individual pallets. Bids work in increments of $50 and only registered users can partake. The website hosts live auctions every Tuesday of the week. First off, you can participate in their live auctions to bid on items alongside other potential buyers. There are three ways you can buy goods from Rogue. Rogue Liquidation works in a partnership with Prime Finds to acquire estate and merchandise from liquidations and close-outs for resale. Ran by a couple of husband and wife, Rogue Liquidation offers you plenty of options to buy items individually or in mass, all at the cheapest prices. Rogue Liquidation is a liquidation that is based in Illinois and has been operating throughout the USA for years now. Related:- Best Liquidation Stores in Chicago Because of the COVID-19 pandemic, they allow customers to book virtual meetings on Zoom as well.Īddress: 3021 W 36th St, Chicago, IL 60632, United States Merchandise USA can be reached through mail, phone, fax, or even a visit to their physical store. You can only place an order that is at least worth $1500 as they do not sell individual items and understandably so. The list of products stretched way too long and it is better to give their website a visit to see if what you want is on there. ![]() from jewelry to hardware to packages and even toys, you can find everything at the lowest prices here. There is a wide variety of liquidated merchandise available for purchase at Merchandise USA. Whether you want to buy merchandise in resale or want to liquidate your assets, they can do it all. The experience and reputation of Merchandise USA are unmatchable and so is the quality of the goods. What began as an individual adventure in the late 1980s, is now a full-fledged business spread over more than 100K square feet all over the country. With over 38 years in the liquidation business, Merchandise USA is one of the most high-profile stores in not only Illinois but the whole of the USA. If you are looking for liquidators in Illinois,īest Liquidation Stores in Illinois: Merchandise USA Liquidated merchandise is cheap by default but one of them is bound to offer deals better than the others. Your set budget can be the best determinant to make your final decision with a liquidation store for buying liquidation pallets in Illinois. Liquidations stores all differ from each other mainly on the basis of how much it costs to buy goods from them. From reviews and testimonials, you can find out the general quality, quantity, and customer service to expect from them. Going for a company with a better reputation should be an easy choice to make. It is crucial to go for an experienced retailer for better value for money. Everyone wants to try their hands at making big money and is getting into the business without any prior knowledge or experience. The whole concept of liquidation resale has kept getting more and more popular as a business idea over the decades. Introducing our new group for Buying and Selling liquidation goods and merchandise!įind exclusive deals and merchandise opportunities! Experience. ![]() ![]() ![]() Still curious? Try the Reddit search features or an advanced Google search, such as " site: /r/boston". All applicants must pass a background check. All applicants must be a resident of New England. should be directed to MA RMV Medical Affairs Office at 85. Questions regarding handicap placards, etc. Remove, clear or accept payment for excise tax issues, parking violations, E-ZPass violations or tickets. Watch out for scammers that flock to tourist locations!įind and share local events with The Boston Calendar All vehicles listed on your MA application must meet the same MA business market requirements for the number of seats, license plates, emissions compliance, etc. Any registration transaction for vehicles in excess of 55,000 pounds. We get a lot of posts asking about visiting and making the most of the city! We ask that you do some research before posting.įor tourism basics, try the official City of Boston Visitor's Guide.įor fun bar, restaurant, and activity suggestions, check out the /r/boston wiki travel page and the wikivoyage Boston page. Youve come to the best place if youre searching for this form. Additionally, as of May 28, 2021, all of Florida's toll roads are compatible with E-ZPass. The E Zpass Ma Application is a great way to pay tolls. Visiting Boston, or seeking locally-sourced tips? States connected to the E-ZPass system include Delaware, Illinois, Indiana, Kentucky, Maine, Maryland, Massachusetts, New Hampshire, New Jersey, New York, North Carolina, Ohio, Pennsylvania, Rhode Island, Virginia and West Virginia. Looking to buy or sell or give away something? See /r/BostonForSale Have renting/owning questions, or just seeking roommate(s)? See /r/bostontenants and /r/bostonhousing/ Looking for a local job? Want to share an opening? See /r/BostonJobs The City of Boston website also has a lot of getting-settled resources. Main Wiki Page Rules Things to See & Do Guide to Moving to Boston Where is my post? Discord Channel Related Subreddits Rules for Journalists QUICK REFERENCE FAQ: Moving to the Boston area?Ĭheck out the moving to Boston Wiki for residency resources and user tips. Low-effort posts will be locked and removed and that doxxing, excessive trolling, witch hunting, brigading, shitstirring, user impersonation and abuse of the report button will not be tolerated under any circumstances.īe civil, don't spam, and follow the rules. so I figured I would throw in my two cents worth (at the current exchange rate. 45, but with a pass from out of state, it’s. To travel the full length of the turnpike with an in-state pass costs. 50, but with a transponder purchased elsewhere, it’s. EZ Pass MA Customer Service Number customer service phone number is 87, address is 27 Midstate Drive, Auburn, Massachusetts 01501, where you can send. We make completing any MA E-Zpass Application for Individual less difficult.Welcome to r/Boston, a reddit focused on the city of Boston, MA and the Greater Boston Area. Answer 1 of 22: There has been quite a slew of questions about EZ pass etc. For example, going from the western-most end of the Mass Pike to the Interstate 495 interchange toward northern New England using a Massachusetts transponder costs. As an instant download, an attachment in an email or through the mail as a hard copy. There are various choices for getting the doc. Select Done in the top right corne to save and send or download the template.Make sure that each and every field has been filled in properly.Encuentre el plan que mejor cumple con sus necesidades. Q: Where should the E-ZPass transponder be installed in my vehicle Q: Can I hold the transponder up, instead of mounting it to my windshield Q: Can vehicles. You will find 3 options typing, drawing, or capturing one. E-ZPass ofrece una variedad de planes de cuentas individuales. Follow the step-by-step instructions below to design your application for individual e pass Massachusetts account massdot state ma: Select the document you want to sign and click Upload. Select the Sign button and make a digital signature.Add the date to the template with the Date feature.Ensure that the data you fill in MA E-Zpass Application for Individual is updated and accurate.Turn on the Wizard mode in the top toolbar to acquire more suggestions.Click the orange Get Form option to begin enhancing. ![]() The following tips, in addition to the editor will assist you through the whole procedure. Some details on other states: - Illinois. E Zpass Ma Customer Service Phone Number: 1-87. A powerhouse editor is right close at hand providing you with an array of advantageous instruments for filling out a MA E-Zpass Application for Individual. Your EZ Pass MA transponder is accepted as a toll payment method at any out of state E-ZPass equipped toll facility. With US Legal Forms the procedure of submitting legal documents is anxiety-free. The times of distressing complex tax and legal documents are over. ![]() ![]() ![]() This is a unique project: price rises are very sensitive for supermarkets because nobody wants to be more expensive than the competition.’Ī spokeswoman for the Dutch Heart Foundation said they had high hopes that if the project shows health improvements, it could encourage wider changes in responsible businesses. ‘This is why we want to make healthier things more attractive and eye-catching, so people are more likely to buy and eat them. ‘I think a lot of people know that vegetables are healthy but there is so much unhealthy food available in the supermarket and you are so easily tempted by all sorts of delicious things,’ said Stuber. They will monitor shopping habits, and via a blood sample, any changes in cholesterol, blood sugar and waist measurement after what might be a year of healthier eating. That’s the nudge.’Īs part of the five-year research project, Supreme Nudge, researchers are looking for 115 regular clients in each supermarket, which are in smaller villages where they are likely to provide the majority of shopping for local residents. Sugar-rich drinks which could sometimes go up in price while drinks with no sugar could sometimes have discounts. ‘The health differences are very large in the Netherlands and we want to do something about it, to make healthier products more attractive and accessible to everyone. ‘People who live in these areas are more likely on average to have diabetes and heart problems.’ ‘The areas that have been chosen have a lower so-called socio-economic position,’ researcher Josine Stuber told. ![]() The project, which unites the Dutch Heart Foundation, health ministry and universities and teaching hospitals including the Amsterdam UMC, aims to do something about the health differences between richer and poorer neighbourhoods, where life expectancy can be six years shorter on average. Four other supermarkets will see no change and will be monitored as controls. A smiley or thumbs-up sticker will point out good choices, which could also be placed in impulse buy racks.Īlthough the supermarket stock will not change overall, in four test supermarkets, prices and locations of products will. The year-long study in eight Coop shops aims to see whether a ‘supreme nudge’ can give consumers the incentive to shop for healthier products and improve their lifestyles and heart health.Ĭosts for products like sugary drinks will go up, while there will be price promotions for healthier products. Tempting chocolate bars by the till will give way to attractively-priced, unsalted nuts as part of a ‘unique’ research project in Dutch supermarkets. Stores to price less healthy food higher for ‘unique’ research project ApPhoto: A tasty snack? Photo: ![]() ![]() ![]() They’re the perfect solution to protect your precious vaporizer from damage. That’s where bags and cases come in handy. ![]() ![]() Since then, they have continued to innovate with the Crafty+ and Mighty+, which have become your trusty on-the-go companions.Īs a responsible vaporizer owner, you know the pain of broken glass, the heartbreaking crunch of a downstem or bowl, and even more traumatic, the loss of your favorite glass piece. They revolutionized the vaporizer market when they rolled out the Volcano in 1996. This way, no one can get into your stash without knowing where the key is!įor marijuana enthusiasts who smoke regularly and don’t know where to store their weed or tools, a stash box is an excellent product to get your hands on.Storz & Bickel has been your go-to brand for over 25 years. If you are highly concerned about security and privacy, choosing a box with a lock or a passcode combination is a much better choice for you. Just leave your tools in there, store it in a drawer or another cool location, and pull it out when you need it! Locking Box Wooden stash boxes look great and are excellent storage spaces for those who aren’t worried about someone getting into their stash. If you’re sold on buying a stash box, there are different types out there that you may be interested in. If you typically keep your weed around your house in different places, consider placing it all within your stash box and pulling it out when you decide that you want to smoke it instead. Why? Well, storing your cannabis in a secure box that keeps your weed away from air and heat will keep your bud fresh for longer. If your stash box is airtight, this is another bonus. Instead, going for a smell-proof box that keeps your weed safe and sound while also trapping the aroma within the box is a far better way to keep your marijuana protected. You can hide your weed, but if the odor manages to leak out of its storage area, it is not really an effective hiding method. If you have to hide your weed, do so with a stash box. A secret stash box is the best way to do this as it is specifically designed for privacy and discreetness. Whether you have children around or friends who tend to go through your stuff, you are going to want to keep your weed hidden and private. What are they? Let’s take a look! Keep Away From Nosy Eyes While this is a relatively straightforward product, there are several benefits that come with using a stash box. Whether you want a specific place to store all these items away or you want to make sure that they are kept away from others, either because they disapprove or you don’t want them to see it, a stash box is a great way to organize and hide your marijuana. Cue the old reliable stash box! If weed storage has been an issue for you in the past, here is a breakdown of what weed stash boxes are and why you should get one of your own.Īptly named, a stash box is a box designed to specifically store away your cannabis and cannabis tools. If you have a family or roommates who are around, you will want to have a safe place to store your weed in. Although marijuana is gradually becoming a more widely-accepted substance as states continue to make progress in its legalization, that doesn’t mean that you just want to leave your weed out for all to see. ![]() ![]() ![]() ![]() Finally, wait for the text to be translated and understand the text in English.Ĭurrently, WeChat Translate only supports Chinese and English, it works similarly to the Waygo App, which was designed to help non-Chinese speakers translate food menus and signs. To use the real-time translator, simply capture an image of anything with either Chinese or English text in it, operating on a point and translate model.įirst, go to the + sign > Scan > Translate, Then take a photo to translate or select a photo from your gallery. This feature can be accessed from the scan feature in the upright corner, which is regularly used to scan QR codes. Scan and TranslateĬan’t read the instructions or menus in China?Ĭhina’s most popular social platform offers AR-based real-time translation. Press and hold on the message you want to translate and then select the right arrow and then press ‘Translate’ and it will automatically translate the message. So, WeChat has added this feature that will translate messages for you. Sometimes you will get messages in Chinese and, unless you can read Chinese characters fluently, this can be a problem. If you want to find something from a specific chat that you can’t find using the general search then you can also enter the chat and press the group information icon (located at the top right of the screen) then select ‘Search History’. Under the ‘Chats’ tab, scroll up and the search bar should appear. The search function can be very useful in lots of different ways! You can use it to search for Official Accounts, articles, moments, chat history, WeChat IDs and more. Go to Me > Settings > Message Notifications > Other Feature Alerts, and finally select ‘Night Mode’. ![]() Then enable Mute Notifications.Īlternatively, you can select ‘Night Mode’ which will turn off all notifications between the hours of 22:00 and 08:00 so you can get a peaceful night’s sleep. Open the chat and select the chat information icon (located at the top right of the screen). What you will quickly discover in WeChat is that you will be included in lots of big groups that, while often very useful, can be very active and leave you with hundreds of notifications if you leave your phone for 10 minutes! So, it is sometimes useful to turn off notifications for certain groups or chats. Handy when you want to enhance your photo, correct a typo, edit text or even adding more photos etc. Click the ‘edit’ link and your original post with be available to edit and update instead of reposting. Simply delete your original post on moments and the banner ‘You deleted a Moments post Edit‘ will appear. The new feature allows you to edit posts that have already been posted. Chengdu Expat has compiled this list of the key and lesser-known hidden Wechat features to help you understand it, as it will quickly become the most important app on your phone. However, if you are new to China, it can be a bit confusing trying to work out all the inner workings and different WeChat features of the app. The social media application provides just about every function you can think of. If you’ve been in Chengdu for two minutes, you’ll have heard about, or already be using, WeChat. ![]() ![]() ![]() Remote PC Access uses the same Citrix Virtual Apps and Desktops components that deliver virtual desktops and applications. For example, virtual desktops or applications and their associated infrastructure. Remote PC Access eliminates the need to introduce and provide other tools to accommodate teleworking. If users can access their office PCs, they can access all the applications, data, and resources they need to do their work. The Citrix platform makes this secure access possible by giving users access to their physical office PCs. Remote PC Access is a feature of Citrix Virtual Apps and Desktops that enables organizations to easily allow their employees to access corporate resources remotely in a secure manner. Questo articolo è stato tradotto automaticamente. (Aviso legal)Įste artigo foi traduzido automaticamente. (Clause de non responsabilité)Įste artículo ha sido traducido automáticamente. (Haftungsausschluss)Ĭe article a été traduit automatiquement. This article has been machine translated.ĭieser Artikel wurde maschinell übersetzt. Questo contenuto è stato tradotto dinamicamente con traduzione automatica. (Aviso legal)Įste texto foi traduzido automaticamente. (Clause de non responsabilité)Įste artículo lo ha traducido una máquina de forma dinámica. (Haftungsausschluss)Ĭet article a été traduit automatiquement de manière dynamique. This content has been machine translated dynamically.ĭieser Inhalt ist eine maschinelle Übersetzung, die dynamisch erstellt wurde. HDX features managed through the registryĬonfigure COM Port and LPT Port Redirection settings using the registryĬonnector for Configuration Manager 2012 policy settingsĬollect a Citrix Diagnostic Facility (CDF) Trace at System StartupĬonfigure with Citrix Analytics for Performance Generic USB redirection and client drive considerationsīest practices, security considerations, and default operationsĬompare, prioritize, model, and troubleshoot policies Monitor, troubleshoot, and support Microsoft Teams HDX video conferencing and webcam video compression GPU acceleration for Windows single-session OS GPU acceleration for Windows multi-session OS Transport Layer Security (TLS) on Universal Print Server Pass-through authentication and single sign-on with smart cards Integrate Citrix Virtual Apps and Desktops with Citrix Gateway Security considerations and best practices Microsoft System Center Configuration Manager environments Microsoft System Center Virtual Machine Manager virtualization environmentsĬitrix Hypervisor virtualization environments Microsoft Azure Resource Manager cloud environments ![]() ![]() ![]() CRE sites are typically found upstream of genes, within the promoter or enhancer regions. The cAMP response element (CRE) is the response element for CREB which contains the highly conserved nucleotide sequence, 5'-TGACGTCA-3’. The protein also has a magnesium ion that facilitates binding to DNA. This chain of leucine residues forms the leucine zipper motif. These leucine residues tightly bind to leucine residues of another CREB protein forming a dimer. Hydrophobic leucine amino acids are located along the inner edge of the alpha helix. When activated, CREB protein recruits other transcriptional coactivators to bind to CRE promoter 5’ upstream region. The following genes encode CREB or CREB-like proteins:ĬREB proteins are activated by phosphorylation from various kinases, including PKA, and Ca 2+/calmodulin-dependent protein kinases on the Serine 133 residue. CREB also has a role in photoentrainment in mammals. CREB downregulation is implicated in the pathology of Alzheimer's disease and increasing the expression of CREB is being considered as a possible therapeutic target for Alzheimer's disease. CREB proteins are expressed in many animals, including humans.ĬREB has a well-documented role in neuronal plasticity and long-term memory formation in the brain and has been shown to be integral in the formation of spatial memory. ĬREB is closely related in structure and function to CREM ( cAMP response element modulator) and ATF-1 ( activating transcription factor-1) proteins. Genes whose transcription is regulated by CREB include: c-fos, BDNF, tyrosine hydroxylase, numerous neuropeptides (such as somatostatin, enkephalin, VGF, corticotropin-releasing hormone), and genes involved in the mammalian circadian clock ( PER1, PER2). CREB was first described in 1987 as a cAMP-responsive transcription factor regulating the somatostatin gene. It binds to certain DNA sequences called cAMP response elements (CRE), thereby increasing or decreasing the transcription of the genes. CREB (top) is a transcription factor capable of binding DNA (bottom) and regulating gene expression.ĬREB-TF (CREB, cAMP response element-binding protein) is a cellular transcription factor. ![]() ![]() ![]() been able to pull it off, this would have been the first (and only) version of Gradius II released for a home console in the U.S. ![]() On a side note, here's an interesting bit of historical trivia for you - had Turbo Technologies Inc. If you have a Duo and are looking for a great shooter, I heartily recommend Gradius II. The extra stage and animated opening are icing on the cake, making an already superb game just that much better. That minor quibble aside, this is the best port of Gradius II that I've ever played. ![]() However, it is present here, and can be annoying at times - once or twice I had to make sure my Duo was still reading the disc (it was) because it was taking so long to load the next stage. ![]() This load time was almost completely eliminated in both Gradius Deluxe Packs, and of course is nonexistant in the actual arcade game. There is noticeable loading time between levels. THere is one minor drawback to this version of Gradius II, and this can be more or less attributed to the limitations of the PC Engine CD-ROM hardware. On hard, Gradius II can certainly be tough. And if you're good enough to complete the game on easy or normal, don't be surprised if all you get is a nice shot of the Vic Viper and a message saying to try the next highest difficulty level - Konami saved the real ending for only the best players. Konami was also nice enough to include a sound test which is accessible through the option menu. The music for this level is also brand new and exclusive to the PC Engine version. Sphinxes attack you, columns try to crush the Vic Viper, and you have to fight a boss that originally appeared in Gradius III. You navigate your way through crumbling ruins that are in the middle of a desert. The stage is reminiscent of both stage five of the NES version of Life Force (the Temple stage) and stage one of Gradius III. Konami also added a new stage that's exclusive to the PC Engine version. If you'd like to see what this intro looks like, check it out below (animated pic courtesy of SHMUPS!). For example, Konami added a PC Engine exclusive animated opening that showcases the Vic Viper and its capabilities - all set to some great Konami hard rock music. However, if you're used to the arcade game, you may be slightly (and pleasantly) surprised by some of the additions Konami made to the PC Engine game. You still have four power-up configurations and two shields to choose from, and all of the arcade levels are intact. Every detail from the arcade game is reproduced perfectly, from the level design to the enemy patterns to the design of the Vic Viper. If you've played the arcade version of Gradius II, then for the most part you know what to expect here. I was able to get a copy of the game in late 1997, and after comparing it to the port found on the Gradius Deluxe Pack and the ROM that can be played via MAME, it stacks up favorably - even surpassing the arcade game in some respects, believe it or not. Konami chose to use the Super CD format when creating the game as well as enhancing the arcade music with RSS Stereo sound, and the end result is nothing short of phenomenal. In 1992, Konami graced the PC Engine with a near arcade-perfect port of Gradius II. I've finished the game numerous times, on varying degrees of difficulty, and I like it more and more each time I play it. It may be tough in spots, true, but in my opinion, it's far from impossible - even with a lack of power ups in certain areas. Much as I hate to disagree with an esteemed colleague of mine (see Kurt Kalata's review of the Gradius Deluxe Pack), I found Gradius II to be one of the most enjoyable chapters in the series. Konami - PC Engine Super CD-ROM² - 1992Įver since I first played it, I've loved the Gradius II arcade game. ![]() |